Abstract: Objective To investigate the epidemic characteristics of astrovirus (HAstrV), sapovirus(SLV), and adenovirus(HAdV) enteric infections that cause acute diarrhea in children in Chongqing. Methods Children younger than  5-year-old with acute diarrhea were recruited in Chongqing in 2014. Fecal samples were detected by nucleic acid tests. The prevalent time of three viruses and the age and gender of infected patients were analyzed. Results In 511 patients (290 males and 221 females), the positive rate of SLV was 4.7% (24/511), in which the dominant strains were GI.1 and GI.2. The positive rate of HAdV was 2.9% (15/511), in which the dominant strains were HAdV-40 and 41. The positive rate of HAstrV was 2.7% (14/511), in which the dominant strain was HAstrV-1. Patients with co-infection were not found. There were no significant differences of the infection rates between females and males in the three types of viruses (P > 0.05). The highest positive rate of SLV was in November and the highest positive rate of HAstrV was in January. The positive rate of HAdV was not different among different months. All of the positive patients were younger than 36 months. SLV infection existed mainly in 13-36 month old children. Conclusions SLV, HAstrV and HAdV are three important pathogens in acute diarrhea in Chongqing. HAdV5, 7 and 31 also cause acute diarrhea, which need long-term monitoring to verify.